PUBLICATIONS
2024
McVeigh BM, De Jesús Pérez JJ, Mihelc EM, Siepe DH, Gogoi P, Mageswaran SK,
Kalocsay M, Moiseenkova-Bell V. Visualization of lysosomal membrane
proteins by cryo electron tomography. Nature Communications. In Review. 2024 Sep 20
Rocereta J.A, Sturhahn T., Pumroy R.A., Fricke T.C., Herzog C., Leffler A., Kalocsay M.,
Moiseenkova-Bell V.Y. Structural insights into TRPV2 modulation by probenecid. Nature
Structural and Molecular Biology, Accepted. 2024 Sep 12
Sun Y, Pumroy RA, Mallik L, Chaudhuri A, Wang C, Hwang D, Danon JN, Goli KD,
Moiseenkova-Bell V, Sgourakis NG. CryoEM structure of an MHC-I/TAPBPR peptide
bound intermediate reveals the mechanism of antigen proofreading. bioRxiv
[Preprint]. 2024 Aug 7:2024.08.05.606663. doi: 10.1101/2024.08.05.606663. PMID:
39211162; PMCID: PMC11361172.
Calvo B, Torres-Vidal P, Delrio-Lorenzo A, Rodriguez C, Aulestia FJ, Rojo-
Ruiz J, McVeigh BM, Moiseenkova-Bell V, Yule DI, Garcia-Sancho J, Patel S,
Alonso MT. Direct measurements of luminal Ca2+ with endo-lysosomal
GFP-aequorin reveal functional IP3 receptors. bioRxiv [Preprint].
2024 Jul 26:2023.07.11.547422. doi: 10.1101/2023.07.11.547422. PMID: 39211134;
PMCID: PMC11360962.
Gallo PN, Mihelc E, Eisert R, Bradshaw GA, Dimek F, Leffler A, Kalocsay M,
Moiseenkova-Bell V. The dynamic TRPV2 ion channel proximity proteome reveals
functional links of calcium flux with cellular adhesion factors NCAM and L1CAM
in neurite outgrowth. Cell Calcium. 2024 Jul;121:102894. doi:
10.1016/j.ceca.2024.102894. Epub 2024 May 4. PMID: 38728789.
Haug FM, Pumroy RA, Sridhar A, Pantke S, Dimek F, Fricke TC, Hage A, Herzog
C, Echtermeyer FG, de la Roche J, Koh A, Kotecha A, Howard RJ, Lindahl E,
Moiseenkova-Bell V, Leffler A. Functional and structural insights into
activation of TRPV2 by weak acids. EMBO J. 2024 Jun;43(11):2264-2290. doi:
10.1038/s44318-024-00106-4. Epub 2024 Apr 26. PMID: 38671253; PMCID:
PMC11148119.
Pumroy RA, De Jesús-Pérez JJ, Protopopova AD, Rocereta JA, Fluck EC, Fricke
T, Lee BH, Rohacs T, Leffler A, Moiseenkova-Bell V. Molecular details of
ruthenium red pore block in TRPV channels. EMBO Rep. 2024 Feb;25(2):506-523.
doi: 10.1038/s44319-023-00050-0. Epub 2024 Jan 15. PMID: 38225355; PMCID:
PMC10897480.
2023
De Jesús-Pérez JJ, Gabrielle M, Raheem S, Fluck EC, Rohacs T, Moiseenkova-
Bell VY. Structural mechanism of TRPV5 inhibition by econazole. Structure. 2024
Feb 1;32(2):148-156.e5. doi: 10.1016/j.str.2023.11.012. Epub 2023 Dec 22. PMID:
38141613; PMCID: PMC10872542.
Sanganna Gari RR, Tagiltsev G, Pumroy RA, Jiang Y, Blackledge M, Moiseenkova-
Bell VY, Scheuring S. Intrinsically disordered regions in TRPV2 mediate protein-
protein interactions. Commun Biol. 2023 Sep 22;6(1):966. doi:
10.1038/s42003-023-05343-7. PMID: 37736816; PMCID: PMC10516966.
Lee BH, De Jesús Pérez JJ, Moiseenkova-Bell V, Rohacs T. Structural basis of
the activation of TRPV5 channels by long-chain acyl-Coenzyme-A. Nat Commun. 2023
Sep 21;14(1):5883. doi: 10.1038/s41467-023-41577-z. PMID: 37735536; PMCID:
PMC10514044.
Van-Duyne G, Blair IA, Sprenger C, Moiseenkova-Bell V, Plymate S, Penning TM.
The androgen receptor. Vitam Horm. 2023;123:439-481. doi:
10.1016/bs.vh.2023.01.001. Epub 2023 Aug 17. PMID: 37717994.
Jones SA, Gogoi P, Ruprecht JJ, King MS, Lee Y, Zögg T, Pardon E, Chand D,
Steimle S, Copeman DM, Cotrim CA, Steyaert J, Crichton PG, Moiseenkova-Bell V,
Kunji ERS. Structural basis of purine nucleotide inhibition of human uncoupling
protein 1. Sci Adv. 2023 Jun 2;9(22):eadh4251. doi: 10.1126/sciadv.adh4251. Epub
2023 May 31. PMID: 37256948; PMCID: PMC10413660.
Feng S, Pumroy RA, Protopopova AD, Moiseenkova-Bell VY, Im W. Modulation of
TRPV2 by endogenous and exogenous ligands: A computational study. Protein Sci.
2023 Jan;32(1):e4490. doi: 10.1002/pro.4490. PMID: 36327382; PMCID: PMC9794027.
2022
Rohacs T, Fluck EC, De Jesús-Pérez JJ, Moiseenkova-Bell VY. What structures
did, and did not, reveal about the function of the epithelial Ca<sup>2+</sup>
channels TRPV5 and TRPV6. Cell Calcium. 2022 Sep;106:102620. doi:
10.1016/j.ceca.2022.102620. Epub 2022 Jul 3. PMID: 35834842.
Pumroy RA, Protopopova AD, Fricke TC, Lange IU, Haug FM, Nguyen PT, Gallo
PN, Sousa BB, Bernardes GJL, Yarov-Yarovoy V, Leffler A, Moiseenkova-Bell VY.
Structural insights into TRPV2 activation by small molecules. Nat Commun. 2022
Apr 28;13(1):2334. doi: 10.1038/s41467-022-30083-3. PMID: 35484159; PMCID:
PMC9051106.
Fluck EC, Yazici AT, Rohacs T, Moiseenkova-Bell VY. Structural basis of
TRPV5 regulation by physiological and pathophysiological modulators. Cell Rep.
2022 Apr 26;39(4):110737. doi: 10.1016/j.celrep.2022.110737. PMID: 35476976;
PMCID: PMC9088182.
2021
Ahmed T, Nisler CR, Fluck EC 3rd, Walujkar S, Sotomayor M, Moiseenkova-Bell
VY. Structure of the ancient TRPY1 channel from Saccharomyces cerevisiae reveals
mechanisms of modulation by lipids and calcium. Structure. 2022 Jan
6;30(1):139-155.e5. doi: 10.1016/j.str.2021.08.003. Epub 2021 Aug 27. PMID:
34453887; PMCID: PMC8741645.
Martino RA, Fluck EC 3rd, Murphy J, Wang Q, Hoff H, Pumroy RA, Lee CY, Sims
JJ, Roy S, Moiseenkova-Bell VY, Wilson JM. Context-Specific Function of the
Engineered Peptide Domain of PHP.B. J Virol. 2021 Sep 27;95(20):e0116421. doi:
10.1128/JVI.01164-21. Epub 2021 Aug 4. PMID: 34346767; PMCID: PMC8475521.
Swanson NA, Lokareddy RK, Li F, Hou CD, Leptihn S, Pavlenok M, Niederweis M,
Pumroy RA, Moiseenkova-Bell VY, Cingolani G. Cryo-EM structure of the
periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution. Mol Cell. 2021 Aug
5;81(15):3145-3159.e7. doi: 10.1016/j.molcel.2021.06.001. Epub 2021 Jul 1. PMID:
34214465; PMCID: PMC8349896.
Moiseenkova-Bell V, Delemotte L, Minor DL Jr. Ion Channels: Intersection of
Structure, Function, and Pharmacology. J Mol Biol. 2021 Aug 20;433(17):167102.
doi: 10.1016/j.jmb.2021.167102. Epub 2021 Jun 11. PMID: 34119491.
Fluck EC, Pumroy RA, Moiseenkova-Bell VY. Production and purification of
TRPV2 and TRPV5 for structural and functional studies. Methods Enzymol.
2021;653:49-74. doi: 10.1016/bs.mie.2021.02.007. Epub 2021 Mar 22. PMID:
34099181; PMCID: PMC8610384.
Conde J, Pumroy RA, Baker C, Rodrigues T, Guerreiro A, Sousa BB, Marques MC,
de Almeida BP, Lee S, Leites EP, Picard D, Samanta A, Vaz SH, Sieglitz F,
Langini M, Remke M, Roque R, Weiss T, Weller M, Liu Y, Han S, Corzana F, Morais
VA, Faria CC, Carvalho T, Filippakopoulos P, Snijder B, Barbosa-Morais NL,
Moiseenkova-Bell VY, Bernardes GJL. Allosteric Antagonist Modulation of TRPV2 by
Piperlongumine Impairs Glioblastoma Progression. ACS Cent Sci. 2021 May
26;7(5):868-881. doi: 10.1021/acscentsci.1c00070. Epub 2021 Apr 14. PMID:
34079902; PMCID: PMC8161495.
Zhao Y, McVeigh BM, Moiseenkova-Bell VY. Structural Pharmacology of TRP
Channels. J Mol Biol. 2021 Aug 20;433(17):166914. doi:
10.1016/j.jmb.2021.166914. Epub 2021 Mar 5. PMID: 33676926; PMCID: PMC8338738.
Asangani I, Blair IA, Van Duyne G, Hilser VJ, Moiseenkova-Bell V, Plymate S,
Sprenger C, Wand AJ, Penning TM. Using biochemistry and biophysics to extinguish
androgen receptor signaling in prostate cancer. J Biol Chem. 2021 Jan-
Jun;296:100240. doi: 10.1074/jbc.REV120.012411. Epub 2021 Jan 9. PMID: 33384381;
PMCID: PMC7949100.
Xu Z, Wise MC, Chokkalingam N, Walker S, Tello-Ruiz E, Elliott STC, Perales-
Puchalt A, Xiao P, Zhu X, Pumroy RA, Fisher PD, Schultheis K, Schade E, Menis S,
Guzman S, Andersen H, Broderick KE, Humeau LM, Muthumani K, Moiseenkova-Bell V,
Schief WR, Weiner DB, Kulp DW. In Vivo Assembly of Nanoparticles Achieved
through Synergy of Structure-Based Protein Engineering and Synthetic DNA
Generates Enhanced Adaptive Immunity. Adv Sci (Weinh). 2020 Feb 27;7(8):1902802.
doi: 10.1002/advs.201902802. PMID: 32328416; PMCID: PMC7175333.
Pumroy RA, Fluck EC 3rd, Ahmed T, Moiseenkova-Bell VY. Structural insights
into the gating mechanisms of TRPV channels. Cell Calcium. 2020 May;87:102168.
doi: 10.1016/j.ceca.2020.102168. Epub 2020 Jan 24. PMID: 32004816; PMCID:
PMC7153993.
2020
Fricke TC, Echtermeyer F, Zielke J, de la Roche J, Filipovic MR, Claverol S,
Herzog C, Tominaga M, Pumroy RA, Moiseenkova-Bell VY, Zygmunt PM, Leffler A,
Eberhardt MJ. Oxidation of methionine residues activates the high-threshold
heat-sensitive ion channel TRPV2. Proc Natl Acad Sci U S A. 2019 Nov
26;116(48):24359-24365. doi: 10.1073/pnas.1904332116. Epub 2019 Nov 12. PMID:
31719194; PMCID: PMC6883831.
Hughes TE, Del Rosario JS, Kapoor A, Yazici AT, Yudin Y, Fluck EC 3rd,
Filizola M, Rohacs T, Moiseenkova-Bell VY. Structure-based characterization of
novel TRPV5 inhibitors. Elife. 2019 Oct 25;8:e49572. doi: 10.7554/eLife.49572.
PMID: 31647410; PMCID: PMC6834369.
Pumroy RA, Samanta A, Liu Y, Hughes TE, Zhao S, Yudin Y, Rohacs T, Han S,
Moiseenkova-Bell VY. Molecular mechanism of TRPV2 channel modulation by
cannabidiol. Elife. 2019 Sep 30;8:e48792. doi: 10.7554/eLife.48792. PMID:
31566564; PMCID: PMC6794088.
Samanta A, Hughes TET, Moiseenkova-Bell VY. Cryo Electron Microscopy of TRP
Channels. Methods Mol Biol. 2019;1987:39-50. doi: 10.1007/978-1-4939-9446-5_3.
PMID: 31028672; PMCID: PMC9526436.
2019
Hughes TET, Pumroy RA, Yazici AT, Kasimova MA, Fluck EC, Huynh KW, Samanta
A, Molugu SK, Zhou ZH, Carnevale V, Rohacs T, Moiseenkova-Bell VY. Structural
insights on TRPV5 gating by endogenous modulators. Nat Commun. 2018 Oct
10;9(1):4198. doi: 10.1038/s41467-018-06753-6. PMID: 30305626; PMCID:
PMC6179994.
Samanta A, Kiselar J, Pumroy RA, Han S, Moiseenkova-Bell VY. Structural
insights into the molecular mechanism of mouse TRPA1 activation and inhibition.
J Gen Physiol. 2018 May 7;150(5):751-762. doi: 10.1085/jgp.201711876. Epub 2018
Apr 27. PMID: 29703838; PMCID: PMC5940248.
Samanta A, Hughes TET, Moiseenkova-Bell VY. Transient Receptor Potential
(TRP) Channels. Subcell Biochem. 2018;87:141-165. doi:
10.1007/978-981-10-7757-9_6. PMID: 29464560; PMCID: PMC6038138.
Basak S, Gicheru Y, Samanta A, Molugu SK, Huang W, Fuente M, Hughes T,
Taylor DJ, Nieman MT, Moiseenkova-Bell V, Chakrapani S. Cryo-EM structure of
5-HT<sub>3A</sub> receptor in its resting conformation. Nat Commun. 2018 Feb
6;9(1):514. doi: 10.1038/s41467-018-02997-4. PMID: 29410406; PMCID: PMC5802770.
Hughes TET, Lodowski DT, Huynh KW, Yazici A, Del Rosario J, Kapoor A, Basak
S, Samanta A, Han X, Chakrapani S, Zhou ZH, Filizola M, Rohacs T, Han S,
Moiseenkova-Bell VY. Structural basis of TRPV5 channel inhibition by econazole
revealed by cryo-EM. Nat Struct Mol Biol. 2018 Jan;25(1):53-60. doi:
10.1038/s41594-017-0009-1. Epub 2018 Jan 1. PMID: 29323279; PMCID: PMC5951624.
2018
Arne JM, Widjaja-Adhi MAK, Hughes T, Huynh KW, Silvaroli JA, Chelstowska S,
Moiseenkova-Bell VY, Golczak M. Allosteric modulation of the substrate
specificity of acyl-CoA wax alcohol acyltransferase 2. J Lipid Res. 2017
Apr;58(4):719-730. doi: 10.1194/jlr.M073692. Epub 2017 Jan 17. PMID: 28096191;
PMCID: PMC5392747.
2017
Huynh KW, Cohen MR, Jiang J, Samanta A, Lodowski DT, Zhou ZH, Moiseenkova-
Bell VY. Structure of the full-length TRPV2 channel by cryo-EM. Nat Commun. 2016
Mar 29;7:11130. doi: 10.1038/ncomms11130. PMID: 27021073; PMCID: PMC4820614.
2016
Cohen MR, Johnson WM, Pilat JM, Kiselar J, DeFrancesco-Lisowitz A, Zigmond
RE, Moiseenkova-Bell VY. Nerve Growth Factor Regulates Transient Receptor
Potential Vanilloid 2 via Extracellular Signal-Regulated Kinase Signaling To
Enhance Neurite Outgrowth in Developing Neurons. Mol Cell Biol. 2015
Dec;35(24):4238-52. doi: 10.1128/MCB.00549-15. Epub 2015 Sep 28. PMID: 26416880;
PMCID: PMC4648816.
2015
Cohen MR, Moiseenkova-Bell VY. Structure of thermally activated TRP
channels. Curr Top Membr. 2014;74:181-211. doi:
10.1016/B978-0-12-800181-3.00007-5. PMID: 25366237; PMCID: PMC4475848.
Huynh KW, Cohen MR, Moiseenkova-Bell VY. Application of amphipols for
structure-functional analysis of TRP channels. J Membr Biol. 2014
Oct;247(9-10):843-51. doi: 10.1007/s00232-014-9684-6. Epub 2014 Jun 4. PMID:
24894720; PMCID: PMC4198461.
2014
Cohen MR, Huynh KW, Cawley D, Moiseenkova-Bell VY. Understanding the
cellular function of TRPV2 channel through generation of specific monoclonal
antibodies. PLoS One. 2013 Dec 31;8(12):e85392. doi:
10.1371/journal.pone.0085392. PMID: 24392006; PMCID: PMC3877370.
Huynh KW, Cohen MR, Chakrapani S, Holdaway HA, Stewart PL, Moiseenkova-Bell
VY. Structural insight into the assembly of TRPV channels. Structure. 2014 Feb
4;22(2):260-8. doi: 10.1016/j.str.2013.11.008. Epub 2013 Dec 26. PMID: 24373766;
PMCID: PMC5548120.
2013
Wang L, Cvetkov TL, Chance MR, Moiseenkova-Bell VY. Identification of in
vivo disulfide conformation of TRPA1 ion channel. J Biol Chem. 2012 Feb
24;287(9):6169-76. doi: 10.1074/jbc.M111.329748. Epub 2011 Dec 29. PMID:
22207754; PMCID: PMC3307328.
Jastrzebska B, Ringler P, Lodowski DT, Moiseenkova-Bell V, Golczak M, Müller
SA, Palczewski K, Engel A. Rhodopsin-transducin heteropentamer: three-
dimensional structure and biochemical characterization. J Struct Biol. 2011
Dec;176(3):387-94. doi: 10.1016/j.jsb.2011.08.016. Epub 2011 Sep 6. PMID:
21925606; PMCID: PMC3210324.
Cvetkov TL, Huynh KW, Cohen MR, Moiseenkova-Bell VY. Molecular architecture
and subunit organization of TRPA1 ion channel revealed by electron microscopy. J
Biol Chem. 2011 Nov 4;286(44):38168-38176. doi: 10.1074/jbc.M111.288993. Epub
2011 Sep 9. PMID: 21908607; PMCID: PMC3207480.
Ludtke SJ, Tran TP, Ngo QT, Moiseenkova-Bell VY, Chiu W, Serysheva II.
Flexible architecture of IP3R1 by Cryo-EM. Structure. 2011 Aug 10;19(8):1192-9.
doi: 10.1016/j.str.2011.05.003. PMID: 21827954; PMCID: PMC3154621.
Moiseenkova-Bell V, Wensel TG. Functional and structural studies of TRP
channels heterologously expressed in budding yeast. Adv Exp Med Biol.
2011;704:25-40. doi: 10.1007/978-94-007-0265-3_2. PMID: 21290288; PMCID:
PMC3615646.
2011
Goc A, Chami M, Lodowski DT, Bosshart P, Moiseenkova-Bell V, Baehr W, Engel
A, Palczewski K. Structural characterization of the rod cGMP phosphodiesterase
6. J Mol Biol. 2010 Aug 20;401(3):363-73. doi: 10.1016/j.jmb.2010.06.044. Epub
2010 Jul 1. PMID: 20600113; PMCID: PMC2917047.
2010
Yao CK, Lin YQ, Ly CV, Ohyama T, Haueter CM, Moiseenkova-Bell VY, Wensel TG,
Bellen HJ. A synaptic vesicle-associated Ca2+ channel promotes endocytosis and
couples exocytosis to endocytosis. Cell. 2009 Sep 4;138(5):947-60. doi:
10.1016/j.cell.2009.06.033. PMID: 19737521; PMCID: PMC2749961.
Moiseenkova-Bell VY, Wensel TG. Hot on the trail of TRP channel structure. J
Gen Physiol. 2009 Mar;133(3):239-44. doi: 10.1085/jgp.200810123. PMID: 19237588;
PMCID: PMC2654091.
2009
Moiseenkova-Bell VY, Stanciu LA, Serysheva II, Tobe BJ, Wensel TG. Structure
of TRPV1 channel revealed by electron cryomicroscopy. Proc Natl Acad Sci U S A.
2008 May 27;105(21):7451-5. doi: 10.1073/pnas.0711835105. Epub 2008 May 19.
PMID: 18490661; PMCID: PMC2396679.
2008
Overexpression and purification of the vanilloid receptor in yeast (Saccharomyces cerevisiae)
Moiseenkova V.Y., Hellmich H., Christensen B.N.
Biochemical and Biophysical Research Communications (2003)